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1.
Astrobiology ; 14(1): 27-32, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24432776

RESUMO

The Mars Science Laboratory (MSL), comprising a cruise stage; an aeroshell; an entry, descent, and landing system; and the radioisotope thermoelectric generator-powered Curiosity rover, made history with its unprecedented sky crane landing on Mars on August 6, 2012. The mission's primary science objective has been to explore the area surrounding Gale Crater and assess its habitability for past life. Because microbial contamination could profoundly impact the integrity of the mission and compliance with international treaty was required, planetary protection measures were implemented on MSL hardware to verify that bioburden levels complied with NASA regulations. By applying the proper antimicrobial countermeasures throughout all phases of assembly, the total bacterial endospore burden of MSL at the time of launch was kept to 2.78×105 spores, well within the required specification of less than 5.0×105 spores. The total spore burden of the exposed surfaces of the landed MSL hardware was 5.64×104, well below the allowed limit of 3.0×105 spores. At the time of launch, the MSL spacecraft was burdened with an average of 22 spores/m², which included both planned landed and planned impacted hardware. Here, we report the results of a campaign to implement and verify planetary protection measures on the MSL flight system.


Assuntos
Contenção de Riscos Biológicos , Meio Ambiente Extraterreno , Laboratórios , Marte , Astronave , Bactérias/isolamento & purificação , Contaminação de Equipamentos
2.
Astrobiology ; 14(1): 33-41, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24432777

RESUMO

On November 26, 2011, the Mars Science Laboratory (MSL) launched from Florida's Cape Canaveral Air Force Station aboard an Atlas V 541 rocket, taking its first step toward exploring the past habitability of Mars' Gale Crater. Because microbial contamination could profoundly impact the integrity of the mission, and compliance with international treaty was a necessity, planetary protection measures were implemented on all MSL hardware to verify that bioburden levels complied with NASA regulations. The cleanliness of the Atlas V payload fairing (PLF) and associated ground support systems used to launch MSL were also evaluated. By applying proper recontamination countermeasures early and often in the encapsulation process, the PLF was kept extremely clean and was shown to pose little threat of recontaminating the enclosed MSL flight system upon launch. Contrary to prelaunch estimates that assumed that the interior PLF spore burden ranged from 500 to 1000 spores/m², the interior surfaces of the Atlas V PLF were extremely clean, housing a mere 4.65 spores/m². Reported here are the practices and results of the campaign to implement and verify planetary protection measures on the Atlas V launch vehicle and associated ground support systems used to launch MSL. All these facilities and systems were very well kept and exceeded the levels of cleanliness and rigor required in launching the MSL payload.


Assuntos
Contenção de Riscos Biológicos , Meio Ambiente Extraterreno , Laboratórios , Marte , Astronave , Bactérias/crescimento & desenvolvimento , Contagem de Colônia Microbiana , Contaminação de Equipamentos , Material Particulado/análise , Estados Unidos , United States National Aeronautics and Space Administration
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